Unit 6: Epithelial Cells - Practice Quiz

BTY114 — Cell Biology Laboratory 60 Questions
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1 Which type of tissue is observed in a human cheek cell preparation?

Observation of human cheek epithelial cells Easy
A. Nervous tissue
B. Epithelial tissue
C. Connective tissue
D. Muscle tissue

2 Which instrument is commonly used to collect cells from the inside of the cheek?

Observation of human cheek epithelial cells Easy
A. Sterile cotton swab
B. Plastic forceps
C. Glass stirring rod
D. Metal ruler

3 Where are cheek epithelial cells collected from?

Observation of human cheek epithelial cells Easy
A. Back of the hand
B. Inside the cheek
C. Surface of the tongue
D. Tip of the nose

4 Why is a stain used when observing cheek cells?

Observation of human cheek epithelial cells Easy
A. To make cells move faster
B. To make cell structures clearer
C. To increase cell size
D. To prevent light from passing

5 Which stain is commonly used to observe the nucleus in cheek cells?

Observation of human cheek epithelial cells Easy
A. Iodine vapor
B. Safranin powder
C. Methylene blue
D. Sudan III

6 Which structure usually appears darker after staining cheek cells?

Observation of human cheek epithelial cells Easy
A. Chloroplast
B. Vacuole
C. Nucleus
D. Cell wall

7 Which microscope part is used to support the glass slide?

Observation of human cheek epithelial cells Easy
A. Fine adjustment knob
B. Eyepiece
C. Diaphragm
D. Stage

8 What is placed over the cheek-cell sample before viewing?

Observation of human cheek epithelial cells Easy
A. Rubber stopper
B. Filter paper
C. Cover slip
D. Test-tube cap

9 What is the main purpose of a cover slip?

Observation of human cheek epithelial cells Easy
A. To stain the sample blue
B. To remove the cell membrane
C. To increase the sample temperature
D. To flatten and protect the sample

10 Which structure controls many activities of a cheek cell?

Observation of human cheek epithelial cells Easy
A. Nucleus
B. Cell membrane
C. Cytoplasm
D. Ribosome

11 Which structure surrounds the cytoplasm of a cheek cell?

Observation of human cheek epithelial cells Easy
A. Chloroplast
B. Cell membrane
C. Capsule
D. Cell wall

12 Which structure fills much of the inside of a cheek cell around the nucleus?

Observation of human cheek epithelial cells Easy
A. Chlorophyll
B. Starch grain
C. Cell wall
D. Cytoplasm

13 Human cheek epithelial cells are classified as which kind of cells?

Observation of human cheek epithelial cells Easy
A. Bacterial cells
B. Animal cells
C. Fungal cells
D. Plant cells

14 Which structure is absent from normal human cheek cells?

Observation of human cheek epithelial cells Easy
A. Cell membrane
B. Nucleus
C. Cell wall
D. Cytoplasm

15 Which objective should usually be used first when focusing a cheek-cell slide?

Observation of human cheek epithelial cells Easy
A. Oil-immersion objective
B. Special dark-field objective
C. Lowest-power objective
D. Highest-power objective

16 What should be done before placing a cheek-cell slide on the microscope stage?

Observation of human cheek epithelial cells Easy
A. Prepare and cover the sample
B. Remove the eyepiece
C. Heat the microscope
D. Paint the slide surface

17 What is the usual shape of a cheek epithelial cell when viewed under a microscope?

Observation of human cheek epithelial cells Easy
A. Flat and irregular
B. Rectangular and green
C. Long and branched
D. Round and thick-walled

18 Which feature distinguishes cheek cells from typical plant cells?

Observation of human cheek epithelial cells Easy
A. They lack chloroplasts
B. They have a rigid cell wall
C. They contain chloroplasts
D. They have a large central vacuole

19 Why should a cheek-cell sample be handled with a clean tool?

Observation of human cheek epithelial cells Easy
A. To increase cell division
B. To reduce contamination
C. To change the cell shape
D. To produce more nuclei

20 Which microscope control is used to make small focusing adjustments?

Observation of human cheek epithelial cells Easy
A. Stage clip
B. Revolving nosepiece
C. Light switch
D. Fine adjustment knob

21 Why is a clean toothpick or sterile swab used to collect cheek cells?

Observation of human cheek epithelial cells Medium
A. To make the nucleus disappear
B. To dissolve the cell membrane
C. To reduce contamination of the sample
D. To increase the cell size

22 What is the main purpose of placing a drop of methylene blue on a cheek-cell preparation?

Observation of human cheek epithelial cells Medium
A. To remove excess cytoplasm from the cells
B. To stain the nucleus for easier observation
C. To increase the magnification of the microscope
D. To make the slide permanently sterile

23 A student sees many irregular, flat, transparent structures after preparing a cheek-cell slide. Which observation best supports that these are cheek epithelial cells?

Observation of human cheek epithelial cells Medium
A. They have visible nuclei after staining
B. They form organized vascular bundles
C. They have rigid cell walls
D. They contain many chloroplasts

24 Why should a coverslip be lowered at an angle onto the cheek-cell sample?

Observation of human cheek epithelial cells Medium
A. To change the cells into a cuboidal shape
B. To increase the cell division rate
C. To prevent or reduce trapped air bubbles
D. To make the nucleus chemically larger

25 A student begins viewing a cheek-cell slide with the objective. What is the most appropriate correction?

Observation of human cheek epithelial cells Medium
A. Remove the coverslip before focusing
B. Begin with the lowest-power objective
C. Use only the fine-adjustment knob immediately
D. Add stain while the objective touches the slide

26 If the eyepiece is and the objective is , what is the total magnification?

Observation of human cheek epithelial cells Medium
A.
B.
C.
D.

27 A cheek cell appears larger when the objective is changed from to . What happens to the field of view?

Observation of human cheek epithelial cells Medium
A. It becomes narrower
B. It becomes wider
C. It becomes completely colorless
D. It remains exactly the same

28 Which structure forms the flexible outer boundary of a human cheek epithelial cell?

Observation of human cheek epithelial cells Medium
A. Large central vacuole
B. Cell membrane
C. Cell wall
D. Chloroplast

29 Why are cheek epithelial cells suitable for a basic microscope investigation?

Observation of human cheek epithelial cells Medium
A. They have unusually thick cell walls
B. They contain visible chloroplasts
C. They are easily collected from the mouth
D. They are naturally arranged in leaf tissue

30 A student observes dark blue spots inside several cheek cells. These spots most likely represent the:

Observation of human cheek epithelial cells Medium
A. Nuclei
B. Extracellular mucus layer
C. Mitochondrial membranes
D. Cell walls

31 What is the most likely result if a cheek-cell smear is too thick?

Observation of human cheek epithelial cells Medium
A. The cells develop rigid walls during observation
B. The microscope automatically increases contrast
C. Every nucleus becomes perfectly transparent
D. Individual cell boundaries become difficult to distinguish

32 Which technique would most likely produce a clearer cheek-cell image?

Observation of human cheek epithelial cells Medium
A. Use a thin smear and adequate stain
B. Use several coverslips at once
C. Leave the slide completely unstained
D. Scrape the cheek repeatedly until tissue clumps

33 Why should a student avoid pressing hard while scraping the inside of the cheek?

Observation of human cheek epithelial cells Medium
A. It causes the microscope lens to lose magnification
B. It prevents methylene blue from staining nuclei
C. It converts epithelial cells into plant cells
D. It may injure the tissue and cause bleeding

34 A student cannot find the cheek cells after switching to high power. What should the student do first?

Observation of human cheek epithelial cells Medium
A. Move the slide rapidly in all directions
B. Remove the objective and clean the specimen
C. Increase the amount of immersion oil
D. Return to low power and relocate the specimen

35 Which feature would distinguish a cheek epithelial cell from a typical onion epidermal cell?

Observation of human cheek epithelial cells Medium
A. The cheek cell is surrounded by cellulose
B. The cheek cell lacks a rigid cell wall
C. The cheek cell has a large central vacuole
D. The cheek cell contains chloroplasts

36 Why might unstained cheek cells be difficult to observe with a light microscope?

Observation of human cheek epithelial cells Medium
A. They have little natural contrast against the background
B. They are too large to fit under a coverslip
C. They reflect all light as a metallic surface
D. They contain no cytoplasm at all

37 A student records that the cheek cells are thin and irregularly shaped. What conclusion is most appropriate?

Observation of human cheek epithelial cells Medium
A. The cells are likely bacterial colonies
B. The cells are consistent with squamous epithelial tissue
C. The cells are specialized for photosynthesis
D. The cells must be conducting nerve impulses

38 If the microscope image moves left when the slide is moved right, what does this demonstrate?

Observation of human cheek epithelial cells Medium
A. The stain has damaged the nucleus
B. The microscope reverses image movement
C. The cells are actively migrating
D. The objective is producing chlorophyll

39 Which observation would provide the strongest evidence that a dark spot is a nucleus rather than debris?

Observation of human cheek epithelial cells Medium
A. It lies within a visible cell boundary
B. It has exactly the same color as the slide
C. It appears only where air bubbles are present
D. It is located outside every cell

40 Why should the microscope slide be labeled before preparing the cheek-cell sample?

Observation of human cheek epithelial cells Medium
A. To make the coverslip adhere chemically
B. To prevent all cells from changing shape
C. To increase the brightness of the lamp
D. To identify the specimen and prevent mix-ups

41 A student observes cheek cells with a compound microscope. The cells appear nearly transparent, and increasing the lamp intensity makes the entire field brighter but does not improve the visibility of the nuclei. Which adjustment would most directly improve nuclear contrast?

Observation of human cheek epithelial cells Hard
A. Adjust the condenser and diaphragm
B. Increase the ocular lens diameter
C. Increase total magnification
D. Use the coarse-adjustment knob

42 A cheek-cell smear contains several overlapping cells, making it difficult to determine individual cell boundaries. Which preparation change would best reduce this problem while preserving the cells for observation?

Observation of human cheek epithelial cells Hard
A. Increase the stain concentration substantially
B. Press the coverslip firmly onto the smear
C. Collect less material and spread it thinly
D. Use the coarse focus at high magnification

43 When switching from a objective to a objective, a student loses the image because the specimen moves out of focus. Which interpretation is most accurate?

Observation of human cheek epithelial cells Hard
A. The ocular lens has produced chromatic aberration
B. The stain has chemically dissolved the cell membrane
C. The microscope has insufficient numerical aperture
D. The microscope is not parfocal or was improperly focused

44 A student records a cheek cell as measuring 58 ocular divisions with a objective. Calibration shows that one ocular division at this objective equals . What is the estimated cell length?

Observation of human cheek epithelial cells Hard
A.
B.
C.
D.

45 A student calculates cheek-cell size using a calibration factor obtained with the objective but applies it to measurements made with the objective. What is the primary consequence?

Observation of human cheek epithelial cells Hard
A. The calculated cells will have correct sizes but wrong colors
B. The calculated cells will have random sizes only
C. The calculated cells will be systematically too large
D. The calculated cells will be systematically too small

46 A cheek-cell preparation shows dark blue granules scattered throughout the cytoplasm, but the nucleus remains poorly defined. Which explanation is most consistent with this observation?

Observation of human cheek epithelial cells Hard
A. The cells were necessarily prokaryotic
B. The specimen contains only cell walls
C. The objective lens lacks magnifying power
D. The cells were stained unevenly or overstained

47 A student sees a round, sharply defined structure in every field, including areas without cells. The structure remains stationary when the slide is moved. What is the most likely identification?

Observation of human cheek epithelial cells Hard
A. A cheek-cell nucleus
B. A mitochondrial cluster
C. A nucleolus in each cell
D. An air bubble under the coverslip

48 A stained cheek cell appears shrunken with a dense, contracted cytoplasmic region separated from the surrounding boundary. Which preparation condition most likely caused this appearance?

Observation of human cheek epithelial cells Hard
A. Exposure to a hypotonic solution
B. Exposure to a hypertonic solution
C. Removal of all extracellular solutes
D. Use of an oil-immersion objective

49 Why is methylene blue particularly useful when observing unstained human cheek epithelial cells?

Observation of human cheek epithelial cells Hard
A. It converts animal cells into rigid polygonal cells
B. It dissolves the plasma membrane to expose organelles
C. It increases nuclear contrast by binding acidic components
D. It prevents all diffusion across the cell boundary

50 A student focuses on a visible dust particle on the coverslip and then moves the slide. The particle does not move with the cells. Which method would best verify that it is not part of the specimen?

Observation of human cheek epithelial cells Hard
A. Switch directly to the highest objective
B. Rotate the ocular lens while holding the slide fixed
C. Move the slide and compare the particle's motion
D. Increase stain exposure until the particle disappears

51 A student reports that cheek epithelial cells possess a cell wall because each cell has a visible outer boundary. Which correction is scientifically appropriate?

Observation of human cheek epithelial cells Hard
A. The boundary is always a cellulose layer
B. The boundary is the stained central vacuole
C. The boundary is formed by bacterial peptidoglycan
D. The boundary is usually the plasma membrane, not a cell wall

52 A student observes many flat, irregularly shaped cells with a single prominent nucleus and no chloroplasts. Which conclusion is best supported by these observations?

Observation of human cheek epithelial cells Hard
A. The specimen likely contains human squamous epithelial cells
B. The cells are skeletal muscle fibers in longitudinal section
C. The specimen definitely contains plant epidermal cells
D. The cells are most likely bacterial colonies

53 Two students prepare cheek-cell slides from the same donor. Student A sees distinct nuclei, whereas Student B sees mostly dark debris. Which variable is the most direct candidate for explaining the difference?

Observation of human cheek epithelial cells Hard
A. Staining time and smear thickness
B. The cells' chromosome number
C. The microscope's brand name alone
D. The donor's blood type alone

54 A cell is visible at the edge of the field under a objective. The student wants to center it before switching to a objective. Which action is most appropriate?

Observation of human cheek epithelial cells Hard
A. Increase illumination until the cell reaches the center
B. Rotate the nosepiece without securing the slide
C. Move the slide so the cell is centered at low power
D. Use the coarse focus while already at high power

55 A student's field becomes darker after switching to a higher-power objective, even though the lamp setting is unchanged. Which explanation is most likely?

Observation of human cheek epithelial cells Hard
A. The ocular lens has reduced specimen thickness
B. The higher-power objective generally transmits less light
C. The cheek cells have stopped absorbing stain
D. The condenser has become a biological structure

56 A cheek-cell image contains a dark crescent along one side of nearly every cell. The crescent changes position when the coverslip is gently shifted. What is the best interpretation?

Observation of human cheek epithelial cells Hard
A. It is evidence that the cells are undergoing mitosis
B. It is a permanent feature of every nucleus
C. It is likely a coverslip-related optical artifact
D. It is necessarily the Golgi apparatus

57 A student wants to compare the average size of cheek cells from two slides. Which procedure would produce the most defensible comparison?

Observation of human cheek epithelial cells Hard
A. Measure random intact cells using the same calibration
B. Estimate size by comparing stain darkness
C. Use different objectives without recalibrating
D. Measure only the largest cells from each slide

58 A cheek-cell smear has numerous dark particles concentrated near the point where the sample was initially placed. Which procedural error most likely produced this pattern?

Observation of human cheek epithelial cells Hard
A. The sample was not spread evenly before staining
B. The eyepiece was cleaned before use
C. The objective was correctly centered
D. The slide was viewed with both eyes open

59 A student identifies a faint circular region inside a stained cheek cell as the nucleolus. Which additional observation would provide the strongest support for that identification?

Observation of human cheek epithelial cells Hard
A. It remains fixed while the slide moves
B. It occurs outside the cell boundary
C. It lies within a larger stained nuclear region
D. It appears in every empty field region

60 A student observes that the apparent thickness of a cheek cell changes dramatically as the fine-focus knob is adjusted. What does this most strongly indicate?

Observation of human cheek epithelial cells Hard
A. The stain is causing reversible chromosome replication
B. The cell is changing shape during every focus adjustment
C. The cell has three-dimensional thickness and limited depth of focus
D. The objective is measuring cell mass directly